Alliance Instruments
Labe (Elbe) Wulkaprodersdorf Agrolinz BALU Eisenstadt Austria Tabak Baxter
Site Products  


Our product is the automation of chemical analysis procedures and the instrument we apply for it.

The reduction of analysis costs, while keeping the quality of analysis results, is a central criterion for the profitability of today's laboratories. Beside detection limits and quality management, the sample throughput, the demand for labour and the instrument maintenance play an important role in the decision process to apply any analysis technique. Automatic chemical analysers provide:

• Five-times the sample throughput in comparison to chromatographic methods

• Small expenses for chemicals, only a fraction of manual method demand is required. Consequently small chemical waste results

• The instrument operation requires no specialist but only trained lab assistants, the automatic analyser saves the stable quality of the determination procedure.

• Important steps of sample preparation are integrated on the analyser. Automatic distillation, UV digestion, dialysis, extraction or integrated dilution save plenty of time.

Detail information about the analysis task is preliminary condition to find the recommendable technical solution. Just the implementation of the objectives makes our automation project competitive and economically interesting. Existing installations may be helpful for the decision of newcomers in automated chemical analysis.



Concept, Application

Specific Advantages

• Specific method on each analysis manifold

• typical 3-4 module / methods

• Expandable up to 12 simultaneous analytical methods

• Usually applied for higher sample throughput and limited number of methods to be automatised

• Unbeatable analysis capacity by simultaneous determination of all applicated methods
• Unique possibility of quality diagnosis, resulting from the use of the segmented flow technique
• High intensity, high repeatable mixing of reaction solutions in each stages of the automated procedure
• Complicated and individual sample preparation and reaction procedures can be applied on the analytic manifold
• Outstanding small running costs, using standard laboratory reagents
• Countless number of available and approved methods, resulting from worldwide use of CFA over decades

Free selection of analysis methods to be measured on each sample of an analysis execution

• Very compact with integrated autosampler and computer

• Usually applied for high analysis capacity for single methods, or for several analysis methods on smaller sample sets

• Outstanding overall repeatability (dilution, sample / reagent transfer, reaction intervals, detection)
• Special "Differential Methods" offer compensation of individual sample colour
• Extremly small demand of sample / reagent, depending on the method and measuring range
• High flexibility, access to method specificatons for adaption
• Simple and safe instrument operation and maintenance
• Integrated self test and operation control
• Excellent relation of cost to performance

• Single parameter or multi parameter (2/4/6) laboratory analyser

• Single parameter or multi parameter
(2-4) online analyser

• Single parameter or multi parameter
(2-4) nutrients-probe  »submergible«

• Sequential analysis or ISE

• Unbeatable sensitivity and stability, thanks to patented LFA technique
• Hermetically close analysis loop
• Compensation of individual sample colour
Complicated and individual sample preparation and reaction procedures can be applied on the analyser
• Extremely small reagent demand and energy consumption, most compact colorimetric analysis components
• Unique nutrients probe analyser (submergible!) thanks to LFA features
• Long term online/in situ autonomy
• Simple operation and maintenance


Continuous-Flow Analysis (CFA)

CFA is also known as Segmented Flow Analysis (SFA), to outline against FIA technique. Existing ISO standards define:   "Methods using flow analysis are automatizing wet chemical procedures and are therefore particularly suitable for processing large sample series... One differentiates between flow injection analysis (FIA) and continuous flow analysis (CFA)."

Continuous Flow Analysis is the traditional technique. We give favour to CFA as it provides valuable advantages for the practical routine work in typical fields of flow analysis application.
Specific advantages

Flowsys 5-channel with xy240

How works CFA analysers ?

Phenolindex NH4 NOx TP Analysis parameter are installed on Analytic Consoles separately. Each sample or calibrant, taken on the autosampler, is splitted to flow into all the analytic modules for simultaneous deter-mination. The aspiration of the subsequent sample after 60 seconds produce an analysis frequency of 60 samples per hour. A sequence of 90 seconds will result in 40 samples per hour throughput. The aspiration of blank water in between is included in this interval.
The gas bubble segmentation of the continuous flowing reaction stream assures best separation between successive samples, also for time consuming preparation steps on the analyser or after long delay for slow reactions. Consequently CFA provides best safety against any memory effect. The number of samples/h multiplied by the number of simultaneous determinations gives the outstanding analysis capacity of analysis, the CFA analyser can carry out.
FLS for nutrient traces
Phenolindex distillation Modern laboratories equipped with latest analytic technology, having no CFA in the past, justify their decision to add CFA technique upon the high efficiency of integrated sample preparation procedures. Automatic digestion of organic P or N compounds, automatic distillation for Cyanide, Phenolindex or Fluorid with 40 samples per hour throughput lead to profitability of investment in acceptable time. Savings of space, glassware handling and washing procedures come together with savings from automatic data documentation
Well experienced users of CFA on the other side purchase new analysers to renew their existing instrumentation, after a working life of 15 years or even more.

PC based system control and data handling with up-to-date Windows software is easy to use, provides multilanguage capability, flexible date export and includes 20 years of experience in specific CFA technique data handling, along several operating systems.
TP with UV digester

Consider, that automatical documentation of original analysis data, integrated QM procedures and computerised calculation and reporting results in additional time savings against manual analysis procedures.


Technical Data of a CFA Analyser:

• Segmented flow techniques Macroflow oder Microflow
• Autosampler serial 52/104 positions or XY. Diluter as option
• Multichannel proportioning pump 12/48 positions, segmentation by air or N2
• Method 'manifold' with individual modules (dilution, incubation, dialysis, distillation,
  UV digestion, hydrolysis, extraction...)
• Individual filter colorimeter for each method module/manifold, lightpath 10-50mm
• Flowcell with mechanic debullbing. Bubble gating as option
• Sophisticated electronics for colorimeter and system control
• PC station with printer. Specific CFA analyser software


Sequential Automatic Analysis (SAA)
(discrete analysis, random access analysis)

Sequential automatic analysis is well established in clinical chemistry, where highly specialised systems execute some hundret samples per hour but are completely unflexible. For our mainly industrial field of activity we offer specific instruments with variable modules and special software, flexible and accessible. The user takes advantage from the reliability and easyness of operation established for clinic purpose.    Specific advantages

How works SAA Analysers ?

The liquid transfer system applies a precision syringe to pipette sample and reagent(s) into the temperature controlled reaction cuvettes. The procedure of liquid transfer assures sufficient mixing and provides safety against carryover. After expiration of specified reaction time for the reagent, a second or third reagent is added, as defined in the method specifications.

After final reaction step the reaction solution is transferred into the optic unit and measured at specific wavelength.

Method procedures using sample blank compen-sation (Differential method) or kinetic methods apply a specific sequence. Reagent blanks measurement always is included in the automatic execution procedure.

To measure low absorbance differences a 40mm Küvette is available.


The sample concentration is calculated considering the value of actual reagent blank and that of measured calibrant solutions. The application of stored calibration factors is possible. Calibration functions can be linear or non-linear.

Sampling unit

Results are stored in the instruments memory, printed locally or transferred to the serial port of a PC (stand alone version).

The use of Easychem for Windows software provides full control of the instrument and data handling via PC.

The automatic analysis procedure includes an instrument self-test on the begin of each execution sequence. Limits can be set in the method specifications for automatic check of results validity. Off values are indicated.

Operators enter the instrument by password identification. Only the master analyst is allowed to access instrument and method settings.

Technical data of a SAA Analyser:

• 60 Positions for samples, calibrant(s), blank
• 18 Positions for reagents, cooled by Peltier
• Pecision syringe 1000µl, resolution 0,3 µl
• 96 Reaction cuvettes, temperatur stabilised 37°C
• Reaction times up to 60 minutes
• Measurement lightpath 7mm, optional 10/40 mm
• Optic unit temperature controlled by Peltier, 8 wavelength available
• 1-8 calibrant per method, alternatively fixed factor in memory
• Measurement procedures Endpoint, Differential, Kinetic
• Automatic dilution of samples and series of calibrants
• Up to 250 determinations per hour with Endpoint / Single Reagent
• Integrated PC, 2x 32 memory positions for methods, *membrane keyboard and
  *built in printer, serial port, ports for external keyboard, printer, *monitor     
  (* depending on the instrument type)
further information


Loop-Flow Analysis (LFA)

The Loop-Flow Analysis was developed in the beginning of the nineties by our partner Systea. LFA is a new and patented technique to carry out colorimetric analysis procedures inside a hermetically closed Loop. Designed with a glance to sea water analysis on board of expeditions, LFA offers several specific advantages. The combination of outstanding stability at lowest concentrations, very compact components and extremely small demand on energy and reagent makes a unique analyser, suitable to solve applications where no convincing solution was available before.

Besides the sequential analysis procedure offers high flexibility to integrate sophisticated sample preparation procedures for laboratory or online instrument version. Within one decade Loop-Flow analysers have gained a deserving and still growing worldwide application.   Specific advantages


How works Loop-Flow Analysis ?

All the steps of a wet-chemical colorimetric analysis method are carried out in an analysis cycle sequentially. The hermetic closed Loop provides full protection against background interference, a basic requirement for stable trace analysis. In the begin of a cycle the loop is washed and filled with sample. The sample colour is measured for compensation. Small amounts of concentrated reagents are added and mixed with high intensity.

This minimum of sample dilution helps to obtain best sensitivity. Along the specified reaction time the solution is temperature controlled if required to speed up or stabilise the reaction. The detection at the specific wavelength is carried out in a double beam colorimeter, the lightpath (5/10/50 mm) is adapted to the application. If automatic dilution is foreseen, a part of the sample is mixed with water in the first steps of the cycle. Upon detection of off-scale concentration a dilution and re-analyse can be carried out automatically.

MICROMAC 1000 - portable analyser

NPR - submergible analyser
The calculation of measured results is based upon the measured and stored values of reagent blank and internal calibrant solution. Results are stored in the instrument memory and sent to *integrated printer or to serial port. Optional modules are available for sample preparation procedures or special reaction steps. Dialysis removes interfering sample matrix by diffusion membrane. UV digestion mineralizes organic P or N compounds. Multi-parameter configurations of LFA analysers are available. The production of working reagents and analyser maintenance is carried out by the user following instructions. Extremely reliable components provide a lifetime of 200.000 analyses.

  Technical data of a LFA Analyser:

Settings for analysis cycle (method) stored in instrument memory
• Membrane keyboard for operation of analysis and access to settings
• LCD display results, alarm and communication menue
• Double-beam colorimeter with monochromatic source, 0.001-1.300 AU
• Automatic range expansion, automatic sample dilution
• Reagent demand about 100µl each analysis
• Energy demand 4W in stand by, about 10W in analysis
• *Industrial design IP65       (* depending on instrument type)
  Detection limit for nutrients:


1 ppb


1 ppb


0,5 ppb


1 ppb

further information

open as pdf

back to top

Service Aktuelles Produkte Anwendungen Kontakt english language Rechtshinweise Allgemeine Geschäftsbedingungen